Table 2.
Virulence factors incorporated in the metabolic network reconstruction of B. cenocepacia J2315
| Virulence factors | Proteins involved | Major metabolic precursors |
|---|---|---|
| Lipopolysaccharide components | ||
| Lipid A | LpxA, LpxB, LpxC, LpxD, LpxH, LpxK, KdsA, KdsB, KdsC, KdtA, KdoO, HtrB | O2, UDP-N-acetyl-D-glucosamine, (R)-3-Hydroxytetradecanoyl-ACP, (R)-3-Hydroxyhexadecanoyl-ACP, Myristoyl-ACP, D-arabinose 5-phosphate, Phosphoenolpyruvate, |
| Core oligosaccharide | GmhA, RfaE, GmhB, HldD, RmlD, WbiI, WaaC, WaaF, WabP, WabR, WabO, WabS, WaaL | Sedoheptulose 7-phosphate, UDP-glucose, UDP-N-acetyl-D-glucosamine, dTDP-4-dehydro-6-deoxy-L-mannose, L-Alanine |
| Ara4N modification | ArnA1, ArnA2, ArnB, ArnC, ArnT | L-Glutamate, 10-Formyltetrahydrofolate, UDPglucuronate |
| Quorum sensing | ||
| AHLs | CepI, CciI | S-adenosyl-L-methionine, Octanoyl-ACP, Hexanoyl-ACP |
| HHQ | KynA, KynB, KynU, PqsA, PqsB, PqsC, PqsD | L-Tryptophan, 3-oxodecanoyl-ACP |
| BDSF | RpfF, FadA, FadB, FadH | (S)-Hydroxydecanoyl-CoA |
| Rhamnolipids | RhlA, RhlB, RhlC, PhaC | dTDP-4-dehydro-6-deoxy-L-mannose, (R)-3-Hydroxydecanoyl-ACP |
Ara4N, 4-amino-4-deoxy-arabinose; AHLs, N-acylhomoserine lactones; HHQ, 2-heptyl-4-quinolone; BDSF, cis-2-dodecenoic acid