Table 5.
Proposed essential enzymes that can be candidate drug targets for B. cenocepacia J2315
| Functional subsystem | EC No. | Protein | Enzyme name |
|---|---|---|---|
| Amino acid metabolism | EC-4.2.3.4 | AroB | 3-dehydroquinate synthase |
| EC-4.2.3.5 | AroC | Chorismate synthase | |
| EC-1.1.1.25 | AroE | Shikimate dehydrogenase | |
| EC-1.3.1.26 | DapB | Dihydrodipicolinate reductase | |
| EC-2.3.1.117 | DapD | Tetrahydrodipicolinate succinylase | |
| EC-5.1.1.7 | DapF | Diaminopimelate epimerase | |
| EC-2.7.2.4 | LysC | Aspartate kinase | |
| Lipid synthesis | EC-6.4.1.2 | AccA* | Acetyl-CoA carboxylase carboxyltransferase subunit-α |
| EC-6.4.1.2 | AccB* | Acetyl-CoA carboxylase biotin carboxyl carrier protein subunit | |
| EC-6.4.1.2 | AccC* | Acetyl-CoA carboxylase biotin carboxylase subunit | |
| EC-6.4.1.2 | AccD* | Acetyl-CoA carboxylase subunit-β | |
| EC-2.7.8.8 | PssA | Phosphatidylserine synthase | |
| Cell wall/LPS synthesis | - | ArnA1# | UDP-Ara4N formyltransferase |
| - | ArnA2# | UDP-4-keto-5-carboxypentose decarboxylase | |
| - | ArnB# | UDP-4-ketopentose aminotransferase | |
| - | ArnC# | Ara4N Und-P transferase | |
| - | ArnT# | Ara4N transferase | |
| EC-3.6.1.27 | BacA | Undecaprenyl pyrophosphate phosphatase | |
| EC-2.5.1.55 | KdsA | 2-dehydro-3-deoxyphosphooctonate aldolase | |
| EC-2.7.7.38 | KdsB | 3-deoxy-manno-octulosonate cytidylyltransferase | |
| EC-2.3.1.129 | LpxA | UDP-N-acetylglucosamine acyltransferase | |
| EC-2.4.1.182 | LpxB | Lipid-A-disaccharide synthase | |
| EC-3.5.1.- | LpxC | UDP-3-O-[3-hydroxymyristoyl]N-acetylglucosamine deacetylase | |
| EC-2.7.1.130 | LpxK | Tetraacyldisaccharide 4'-kinase | |
| EC-2.5.1.7 | MurA* | UDP-N-acetylglucosamine 1-carboxyvinyltransferase | |
| EC-1.1.1.158 | MurB | UDP-N-acetylmuramate dehydrogenase | |
| EC-6.3.2.8 | MurC | UDP-N-acetylmuramate--L-alanine ligase | |
| EC-6.3.2.9 | MurD | UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase | |
| EC-6.3.2.13 | MurE | UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase | |
| EC-2.4.1.227 | MurG | Undecaprenyldiphospho-muramoylpentapeptide-β-N-acetylglucosaminyltransferase | |
| EC-1.1.1.22 | Udg# | UDP-glucose dehydrogenase | |
| EC-2.4.1.- | WaaF | UDP-glucose:(heptosyl) LPS-α-1,3-glucosyltransferase | |
| EC-5.1.3.13 | RmlC | dTDP-4-dehydrorhamnose 3,5-epimerase | |
| Vitamin and cofactor synthesis | EC-2.7.11.5 | AceK | Bifunctional isocitrate dehydrogenase kinase/ phosphatase protein |
| EC-4.1.2.25 | FolB | Dihydroneopterin aldolase | |
| EC-2.5.1.15 | FolP* | Dihydropteroate synthase | |
| EC-1.2.1.70 | HemA | Glutamyl-tRNA reductase | |
| EC-2.1.2.11 | PanB | 3-methyl-2-oxobutanoate hydroxymethyltransferase | |
| EC-6.3.2.1 | PanC | Pantoate--β-alanine ligase | |
| EC-1.1.1.169 | PanE | 2-dehydropantoate 2-reductase | |
| EC-3.1.3.1 | PhoA* | Alkaline phophatase | |
| EC-3.5.4.25 | RibB | Bifunctional 3,4-dihydroxy-2-butanone 4-phosphate synthase | |
| EC-3.5.4.26 | RibD | Riboflavin-specific deaminase/reductase | |
| EC-2.5.1.9 | RibE* | Riboflavin synthase subunit-α | |
| EC-2.5.1.9 | RibH* | 6,7-dimethyl-8-ribityllumazine synase |
# Essential enzymes unique for B. cenocepacia J2315 (others are shared between B. cenocepacia J2315 and P. aeruginosa PAO1).
* The proteins have had approved drugs from the DrugBank database.