Figure 1.

Schematic representation of the sensitization and challenge in this study. Groups of mice (n = 6) were daily intragastically (i.g.) sensitized with 30 mg or 80 mg freshly homogenized rice flour. Challenges were performed at day 4, 7, 11, 14, 18, 21, 25, and 28 after sensitization in each experimental group. Before each challenge, mice were bled from the submandibular veins. The symptoms of hypersensitivity were recorded 1-1.5 h after challenge. Vascular leakages were detected before the 6th challenge. The mice were sacrificed 4 days after the last challenge (day 28), and histopathological analysis was performed. The control group mice were orally sensitized and challenged with PBS.