Table 1.

PCA reactions after injection of undiluted pooled sera

Donor sensitizaion	Heat inactivation	Diameter (mm) mean ± SD	Positive reaction sites n/total
RP, 30 mg	-	5.32 ± 0.83*	12/12
RP, 80 mg	-	6.96 ± 0.35***	12/12
RP, 80 mg	+	0.13 ± 0.10	0/6
PBS		0.12 ± 0.10	0/12

Pooled sera from mice sensitized with RP were injected intradermally into naïve BALB/c mice (n = 2 to 4) including three antigen and one control injection sites for each mouse. Injection of PBS served as sham control. A positive reaction refers to the skin at the injection sites with bluing greater than 3 mm in diameter. * P < 0.01 versus PBS sham control or heated immune sera. ** P < 0.01 versus sera from 30 mg RP daily sensitized mice group. All detections were repeated twice.