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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: J Nutr Biochem. 2011 Mar 22;23(1):93–100. doi: 10.1016/j.jnutbio.2010.11.012

Fig. 2.

Fig. 2

A. γ-T3 treatment of MCF-7 cells causes a modest G-1 arrest. MCF-7 cells were treated with DMSO control (white bars) 40 μM of γ-T3 (black bars) for 24 h and cell cycle distribution was determined by flow cytometry. (representative of 3 experiments, n=3, P<0.05). B. Effects of γ-T3 on cell cycle regulatory proteins. C. γ-T3 induces apoptosis in MCF-7 and MDA-MB 231 cells. Expression of cell cycle regulatory proteins, Cyclin D1, D3, CDK4 and apoptosis indicators, cleaved-PARP and cleaved-caspase 7 was determined by immunoblotting. β-Actin and GAPDH antibodies were used as loading control.