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. 2011 Jun;80(5):1241–1259. doi: 10.1111/j.1365-2958.2011.07640.x

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Copyright © 2011 Blackwell Publishing Ltd

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

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Fig. 2 — Lipoglycan profiles of wild-type and mutant strains of C. glutamicum.

A. The left-hand and middle panels illustrate lipoglycans extracted from wild-type, mutant and complemented strains analysed using SDS-PAGE and visualized using a Pro-Q emerald glycoprotein stain (Invitrogen). The right-hand panel illustrates purified Cg-LAM and Cg-LM from C. glutamicumΔmptC and C. glutamicumΔmptD, respectively, in comparison with wild-type C. glutamicum lipoglycans. Lipoglycan profiles are represented with standard molecular weight markers of glycoproteins of 180, 82, 42 and 18 kDa.

B. MALDI-TOF-MS spectra of Cg-LM and Cg-LAM from different strains of C. glutamicum. MALDI-TOF-MS spectra were acquired in the linear negative mode with delayed extraction using 2,5-dihydrobenzoic acid as a matrix.