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. 2011 Jun;80(5):1241–1259. doi: 10.1111/j.1365-2958.2011.07640.x

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Copyright © 2011 Blackwell Publishing Ltd

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

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Fig. 3 — Glycosyl linkage analysis of Cg-LM and Cg-LAM from different strains of C. glutamicum.

A. Cg-LM per-O-methylated samples were hydrolysed using 2 M TFA, reduced and per-O-acetylated. The resulting partially per-O-methylated, per-O-acetylated alditol acetates were analysed using GC-MS.

B. Cg-LAM per-O-methylated samples were hydrolysed using 2 M TFA, reduced and per-O-acetylated. The resulting partially per-O-methylated, per-O-acetylated alditol acetates were analysed using GC-MS. The partially per-O-methylated, per-O-acetylated alditol acetates were identified based on their characteristic alditol cleavage acetal profiles: t-Araf (m/z 118, 161), t-Manp (m/z 118, 102, 145, 161, 162, 205), 2-Manp (m/z 129, 130, 161, 190), 6-Manp (m/z 118, 129, 162, 189, 233) and 2,6-Manp (m/z 129, 130, 189, 190).