Lipoglycan analysis of C. glutamicumΔmptCΔmptD.
A. Lipoglycans were extracted from wild-type and mutant strains and were analysed using SDS-PAGE and visualized using a Pro-Q emerald glycoprotein stain (Invitrogen). Lipoglycan profiles are represented with standard molecular weight markers of glycoproteins of 180, 82, 42 and 18 kDa.
B. 2D 1H-13C-NMR HMQC characterization of Cg-LAM from C. glutamicum as described in Tatituri et al. (2007b).
C. 2D 1H-13C-NMR HMQC characterization of Cg-LAM from C. glutamicumΔmptCΔmptD. NMR spectra of Cg-LAMs were recorded in D2O at 313 K.