Fig. 8.

TLR-2 stimulation by wild-type and mutant Cg-LM and Cg-LAM. HEK293 cells transfected with TLR2 were stimulated with 50 µg ml⁻¹ of wild-type (WT) or mutant (ΔmptC/ΔmptD) Cg-LAM or Cg-LM. Unstimulated cells (−) served as a control. After 24 h of stimulation (37°C), supernatants were harvested and analysed for IL-8 content using ELISA. The results represent the mean IL-8 production ± SD from three independent experiments (each experiment was performed in triplicate). Double asterisks indicate highly significant (P < 0.001) differences as compared to the unstimulated control cells.