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. 2011 Jun 22;31(25):9093–9100. doi: 10.1523/JNEUROSCI.0550-11.2011

Figure 1.

Figure 1.

Activation of CaMKII in synaptic boutons. A, Pseudo-color image showing the change in the CFP/YFP FRET ratio in a type Ib bouton induced by nerve stimulation for 15 s at 70 Hz. An increase in CaMKII activity is shown as a shift to warmer colors. Scale bar, 2 μm. B, Quantification of FRET responses to stimulation for 15 s at 70 Hz in control boutons (Con) (n = 8), control boutons treated with 10 μm KN92 (n = 5), 10 μm KN93 (n = 9), or 20 nm PLTX (n = 6), and in T305/306D mutants (n = 6). *p < 0.05, ***p < 0.001. C, FRET responses to 70 Hz nerve stimulation with 100 μm ryanodine (Ryan) (n = 6) or expression of RyR-targeted RNAi constructs RyR109631 (n = 7) and RyR10844R-3 (n = 11). Note that an increase in the plotted ratio corresponds to activation.