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. 2009 Dec 21;25(5):1138–1146. doi: 10.1002/jbmr.5

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Copyright © 2010 American Society for Bone and Mineral Research

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Fig. 1 — Thyroid hormone treatment increases Igf1r expression in rat growth plate chondrocytes. (A, C) Quantitative real-time RT-PCR analysis of Igf1r and Igf1 mRNA expression in pellet cultures of growth plate chondrocytes treated with 100 ng/mL of T₃ for 1 to 8 days. The expression in T₃-treated cells was normalized to the expression in control cells. (B) Immunoblotting of IGF1R protein from whole-cell lysates of growth plate chondrocytes treated with or without T₃ (100 ng/mL) for 2 to 7 days. Actin was used as an internal control. (D) Detection of IGF-1 protein in culture medium of growth plate chondrocytes treated with or without T₃ for 1 to 8 days. (E, F) Cyclin D1 mRNA and BrdU incorporation in growth plate cells treated with or without T₃ for 1 to 8 days. (G, H) Col10a1 mRNA and alkaline phosphatase activity in growth plate chondrocytes treated with or without T₃ for 1 to 8 days. *p < .05 versus control cells.