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. 2009 Dec 21;25(5):1138–1146. doi: 10.1002/jbmr.5

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Copyright © 2010 American Society for Bone and Mineral Research

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Fig. 3 — IGF-1/IGF1R promotes Wnt/β-catenin signaling, cell proliferation, and terminal differentiation of growth plate chondrocytes. (A) Quantitative RT-PCR analysis of Wnt4 mRNA expression in rat growth plate chondrocytes infected with Ad-IGF1R or control adenovirus at an MOI of 50 and treated for 5 days with or without IGF-1 (50 ng/mL). (B) Immunoblotting of β-catenin from whole-cell lysates of growth plate chondrocytes treated with or without Ad-IGF1R and IGF-1 for 5 days. Actin was used as an internal control. (C, D) Runx2 mRNA and cyclin D1 mRNA expression in growth plate chondrocytes treated with Ad-IGF1R and/or IGF-1 for 5 days. (E) BrdU incorporation in growth plate chondrocytes treated with Ad-IGF1R and/or IGF-1 for 5 days. (F) Col10a1 mRNA in growth plate chondrocytes treated with Ad-IGF1R and/or IGF-1 for 8 days. The data were expressed as the fold increase over the results of the cells infected with control adenovirus. *p < .05 versus control cells.