Fig. 3.

Confirmation of knockdown of wuc in testes. A) We used quantitative RT-PCR of testis RNA samples to determine the knockdown of wuc mRNA in testes, by comparing the wuc signal to that of a control gene, CG18628, which is expressed in terminal epithelium (www.fly-ted.org). At 29 °C flies with one copy of the UAS-wucRNAi construct and one of the driver (bam-Gal4VP16, BG4) had about 9% of wild type wuc mRNA levels. This was reduced to ~ 3% in flies with two copies of the driver. Expression of a lin-52 RNAi construct had no effect on wuc mRNA levels in testes. B) Western blotting confirms the depletion of Wuc protein in wucRNAi testes. Control driver-only or UAS-RNAi-only testes had normal levels of Wuc protein.