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. 2011 Jun;13(6):836–845. doi: 10.1111/j.1462-5822.2011.01582.x

Fig. 5.

Fig. 5

Effects of PAK1 inhibitors on MEK1 phosphorylation and phosphorylation status of host erythrocyte PAK1 in P. falciparum-infected cells.

A. Effect of the IPA-3 PAK1 inhibitor on MEK1 Ser-297 phosphorylation. Synchronous P. falciparum cultures were treated at ring stage with 15 µM IPA-3 (or with the DMSO vehicle only) for 27 h prior to Western blot analysis using the antibodies indicated to the right. URBC, uninfected red blood cells.

B. Increased phosphorylation of PAK1 Ser-144 in iRBCs. A Western blot analysis was performed on protein extracts from in vitro cultured infected (I) or uninfected (U) RBC ghosts using anti-PAK1 antibody (Cell Signaling; top panel) or anti-phospho-PAK 1/2/3 (Ser-144) (Invitrogen; bottom panel). Lane C (positive control) contains an extract of A673 cells (Santa Cruz).