Figure 2.

Identification of β-thalassemia genotypes using the MeltPro HBB assay. The negative derivative fluorescence over temperature versus temperature was plotted for each sample. Melting curves and corresponding genotypes (box) of the 24 mutations plus wild-type alleles are given according to tube 1 (A) and tube 2 (B). The four detection channels are shown by the corresponding fluorophores. Melting curves of HEX and CY5 channels in tube 1 and of HEX and ROX channels in tube 2 are separated into two panels. Black lines indicate no-template control.