Table 2.
Calibrated Tm of 24 Mutations Detected Using the MeltPro HBB Assay
| Tube | Color | Genotype | Tm (°C) | ΔTm (3 SD) (°C)⁎ |
|---|---|---|---|---|
| 1 | FAM | Wild-type | 65.9 | NA |
| c.316-197C>T | 59.9 | 6.0 (0.42) | ||
| HEX | Wild-type | 65.4 | NA | |
| c.-78A>G | 57.0 | 8.4 (0.39) | ||
| c.-79A>G | 58.4 | 7.0 (0.54) | ||
| c.-80T>C or c.-81A>C† | 62.1 | 3.3 (0.42) | ||
| c.-82C>A | 54.3 | 11.1 (0.39) | ||
| ROX | Wild-type | 67.7 | NA | |
| c.45-46insG | 66.0 | 3.1 (0.45) | ||
| c.48-49insG | 66.6 | 1.7 (0.35) | ||
| c.52A>T | 62.2 | 5.5 (0.54) | ||
| Cy5 | Wild-type | 67.1 | NA | |
| c.79G>A | 61.6 | 5.4 (0.31) | ||
| c.92 + 1G>T or c.92 + 5G>C† | 63.4 | 3.7 (0.33) | ||
| c.91A>G | 59.0 | 7.9 (0.51) | ||
| 2 | FAM | Wild-type | 68.4 | NA |
| c.79G>A | 61.4 | 7.0 (0.36) | ||
| c.84-85insC | 64.0 | 4.4 (0.45) | ||
| HEX | Wild-type | 71.0 | NA | |
| c.124-127delTTCT | 60.1 | 10.8 (0.54) | ||
| c.115delA | 61.3 | 9.7 (0.48) | ||
| c.130G>T or c.113G>A† | 66.3 | 4.7 (0.48) | ||
| ROX | Wild-type | 68.1 | NA | |
| c.216-217insT | 63.8 | 4.3 (0.54) | ||
| c.216-217insA | 62.5 | 5.6 (0.50) | ||
| c.315 + 2delT | 48.1 | 20.0 (0.45) | ||
| c.315 + 5G>C | 51.4 | 16.7 (0.24) | ||
| Cy5 | Wild-type | 64.6 | NA | |
| c.-123A>T | 58.1 | 6.5 (0.42) | ||
| c.-140C>T | 55.7 | 8.9 (0.33) |
NA, not applicable.
⁎
ΔTm = Tm (wild-type) − Tm (mutant).
†
These mutations are judged to be the same type within pairs using the MeltPro HBB assay.