Figure 2.

RREB1 transcripts in bladder tumors and cancer cell lines. A: mRNA expression of total RREB1, RREB1α, and RREB1β was measured in bladder and prostate cancer cell lines. RNA quantities cannot be compared among total RREB1, RREB1α, and RREB1β, because the levels were calculated on unique standard curves for each primer pair. B: Total RREB1 expression was measured in paired normal and cancer (tumor) urothelial bladder (BL) and prostate (PR) tissues, using primers that span exons 2 and 3 of RREB1, an area lacking alternative splicing. Arrows indicate samples in which RREB1 expression is greater in the tumor than in the paired normal tissue. C and D: RNA expression of the RREB1α and RREB1β splice variants was measured using primers targeting base pairs at unique splice junctions.