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. 2011 Jul;179(1):477–486. doi: 10.1016/j.ajpath.2011.03.038

Figure 5.

Figure 5

Depletion of RREB1β decreases UMUC-3 growth in vitro. A: UMUC-3 cells were treated with 25 nmol/L siRNA of control (GL2), RREB1 (all isoforms), and three unique siRNAs targeting exon 8 (8-1, 8-2, and 8-3). Growth was measured 96 hours after siRNA depletion using Alamar Blue and normalized to the GL2 control. B: Real-time PCR for the RREB1α splice variant was performed for the experiment described in panel A to confirm knockdown of RREB1α. RNA levels were normalized to the GL2 control siRNA. C: UMUC-3 cells were treated with 25 nmol/L siRNA of control (GL2), RREB1 (all isoforms), and three unique siRNAs targeting exon 9 (9-1, 9-2, and 9-3). D: RREB1α and RREB1β-specific real-time PCR was used to confirm RREB1α and RREB1β knockdown.