Table 3.
Comparison of the Prostatic Carcinoma Phenotypes Derived from Orthotopically Transplanted Clone 1 and Clone 2 Cells
| Tumor histology | Clone 1 |
Clone 2 |
|
|---|---|---|---|
| Adenosquamous carcinoma |
Sarcomatoid > adenocarcinoma | ||
| +Androgen | −Androgen | ||
| Frequency of lung metastasis | 7/8 | 9/9 | 1/12 |
| Phenotype of metastasis | N/A | ||
| Luminal phenotype | 5/5 | 3/5 | |
| CK8+/CK5(+/−)/TP63− | |||
| Biphenotypic | 2/5 | 4/5 | |
| CK8+/CK5+/TP63+ | |||
| Osseous/cartilagenous metaplasia | 0/17 | 11/12 | |
Ten mice orthotopically injected with Clone 1 cells were selected for immunophenotyping of lung metastases. Lung metastases were scored as being of luminal phenotype (all cells in metastasis contain CK8 and/or CK5 and are negative for TP63) or as biphenotypic (all cells in metastasis contain CK8 and/or CK5 and at least one cell is positive for TP63); > = in Clone 2 orthotopic tumors, sarcomatoid carcinoma was the dominant histological pattern; +Androgen = subcutaneous androgen pellet was replenished at week 7 after orthotopic injection; −Androgen = subcutaneous androgen pellet was removed at week 7 after orthotopic injection.
N/A = not available (one mouse orthotopically injected with Clone 2 cells had a single small lung metastasis that did not appear in all three serial sections that were needed for immunophenotyping).