Figure 11.

Time-course effects of Aβ_25-35 (10 μg/rat) intracerebroventricular injection on the microglial reaction using Iba-1 immunolabeling in the frontal cortex (A), hippocampus CA1, CA3, and DG subfields (B), parietal cortex (C), amygdala (D), and hypothalamus paraventricular nucleus (E) determined in untreated rats [control group (C)] and at 1, 2, and 3 weeks after Aβ_25-35 injection. The injection of the Aβ_25-35 scrambled peptide (10 μg/rat i.c.v.) served as negative control and induced no modifications of the Iba-1 signal. Activated microglia were visualized with Alexa Fluor 488-labeled specific antibody against Iba-1 (green); the nucleus was counterstained with DAPI (blue). In A, asterisks indicate matching locations in corresponding images presented at both lower and higher magnification. CAn, hippocampal subfields; Ce, central amygdaloid nucleus; DG, dentate gyrus; m, magnocellular; p, parvocellular; PVN, paraventricular hypothalamic nucleus; 3v, third ventricle. Scale bars = 100 μm.