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. 2011 Jul;179(1):315–334. doi: 10.1016/j.ajpath.2011.03.021

Figure 2.

Figure 2

A: Electron microscopy of Aβ25-35-HLF peptide after in vitro incubation at 37°C for 4 days. Aβ25-35-HLF formed an extensive network of fibrils after 4 days at 37°C. Scale bar = 120 nm. B: Congo Red staining of Aβ25-35-HLF peptide preaggregated in vitro at 37°C for 4 days. Green birefringence was observed in preaggregated Aβ25-35-HLF. C–Y: Localization within brain structures of Aβ25-35-HLF, determined at 1, 3, and 12 hours, 1 and 3 days, and 1, 2, and 3 weeks after intracerebroventricular injection. Aβ25-35-HLF was visualized in green; the nucleus was counterstained with DAPI (blue). alv, alveus of the hippocampus; Arc, arcuate hypothalamic nucleus; B, basal nucleus of Meynert; bv, blood vessel; CA1, field CA1 of hippocampus; CA3, field CA3 of the hippocampus; cc, corpus callosum; Ce, central amygdala nucleus; cl 10, cerebellar lobule 10; Cx, cerebral cortex; D3V, dorsal third ventricle; DG, dentate gyrus; ec, external capsule; fi, fimbria of the hippocampus; LSD, lateral septal nucleus–dorsal part; LSI, lateral septal nucleus–intermediate part; LV, lateral ventricle; MEE, median eminence–external part; MEI, median eminence–internal part; MHb, medial habenular nucleus; MVe, medial vestibular nucleus; np, needle path; ox, optic chiasma; PeVN, periventricular hypothalamic nucleus; PVN, paraventricular hypothalamic nucleus; PVP, paraventricular thalamic nucleus–posterior part; vhc, ventral hippocampal commissure; 3V, third ventricle; 4V, fourth ventricle. Arrowheads indicate blood vessels. Scale bars = 100 μm.