Angiogenic response induced by rAAV1-Flk-sel treatment. A–F: IHC in the glomerulus (A–D) and tubulointerstitium (E and F) for thrombomodulin (black arrows in A, B, E, and F), BrdU (arrow in C) and double staining of thrombomodulin (black arrows) with BrdU (white arrow) (D) at 1 month after UNx in eNOSKO mice treated with rAAV1-EV (A and E) or with rAAV1-Flk-sel (B, C, D, and F). Scale bars = 20 μm. G–K: Quantitative analysis for thrombomodulin expression (G and J) and number of BrdU-positive cells (H and K) in the glomerulus (G and H) and tubulointerstitium (J and K), and for double staining of thrombomodulin with BrdU in the glomerulus (I). *P < 0.01, **P < 0.001, and ***P < 0.05. Data are expressed as means ± SD; n = 10 in each group.