Figure 1.
RelB recombinant adenovirus delivery by intranasal aspiration induces RelB overexpression in mouse lungs. A: Overexpression of human RelB was induced with an RelB recombinant adenovirus delivered by intranasal aspiration [two doses of 5 × 108 plaque-forming units (pfu) per mouse delivered 24 hours apart], and RelB expression was detected in whole mouse left lung homogenates using Western blot 3, 5, and 7 days after the first adenovirus dose. Saline indicates saline treated; Control, control virus treated (two doses of 5 × 108 pfu per mouse delivered 24 hours apart, both 7 days after initial treatment). B: Densitometric analysis revealed sevenfold, ninefold, and fivefold increases in RelB expression 3, 5, and 7 days, respectively, after the first treatment. RelB overexpression was confirmed 7 days after the initial treatment by IHC with an antibody specific to the human RelB isoform. C: Control virus. D: RelB virus. n = 3 for each group except saline treated, for which n = 1. *P < 0.05 for statistical significance relative to the control-treated mice (one-way analysis of variance with a Tukey posttest).