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. 2011 Jul;179(1):134–140. doi: 10.1016/j.ajpath.2011.03.006

Figure 5.

Figure 5

JUND knockdown in conditionally immortalized human podocyte cell lines. The human podocyte cell line was incubated for 14 days at 37°C to allow full differentiation and maturation. A: The difference in cell morphological features between the undifferentiated (33°C, top) and differentiated (37°C, bottom) podocytes is illustrated by light microscopy. B:VEGFA and JUND expression assessed by RT-qPCR in undifferentiated and differentiated podocytes. C: Knockdown with JUND siRNA (100 nmol/L) in differentiated podocytes showed 80% knockdown compared with scrambled siRNA (control siRNA, 100 nmol/L) by RT-qPCR. Both VEGFA and HIF1A expression levels were significantly increased when JUND expression was knockdown. *P < 0.05, **P < 0.01.