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. 2011 Jun 22;100(12):2981–2990. doi: 10.1016/j.bpj.2011.05.005

Figure 1.

Figure 1

Illustration of the FCCS assays used in this study. (A) Incorporation assay. An intracellular FCCS assay was used to study the incorporation of the guide strand. The incorporation of the labeled guide strand results in increased cross-correlation amplitudes (red curve), whereas the incorporation of the nonlabeled passenger strand results in low cross-correlation amplitudes (black curve). (B) Target interaction assay. An intracellular FCCS assay was used to study the interaction of EGFP-RISC with its fluorescently labeled target RNA. A stable interaction can be observed for miRNAs that do not display RNA-cleavage activity, resulting in increased cross-correlation values (red curve), whereas no stable interaction can be observed for RISC complexes loaded with a guide RNA showing no sequence similarities or a guide strand leading to RISC-mediated target RNA cleavage (black curve).