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. 2011 Jun 22;100(12):2981–2990. doi: 10.1016/j.bpj.2011.05.005

Figure 2.

Figure 2

Effects of 3′-2′-OMe modifications on silencing, guide-strand incorporation, strand separation, and asymmetry. (A) Illustration of the siTK3 siRNA with 2′OMe nt positions of the various modified strands. ER293 cells were transfected with the indicated amounts of differently modified siTK3 together with the fixed concentration of the pGL2-Control and pRL-TK reporter plasmids. After 48 h, the ratios of target to control luciferase concentration were normalized to the NegsiRNA control (black bar). Unmodified siTK3, lined bar; siTK3 3′4nt, light gray; siTK3 3′6nt, gray; siTK3 3′8nt, dark gray; siTK3 3′10nt, open bar. The plotted data were averaged from six independent experiments ± SD. (B) ER293 cells were transfected with the indicated amounts of siTK3 modified on the 3′-end with either 4 or 8 2′-OMe nt as illustrated in the graph. After 48 h, the ratios of target to control luciferase concentration were normalized to the NegsiRNA control. The plotted data were averaged from six independent experiments ± SD. (C and D) Cross-correlation amplitudes in the cytoplasm (solid bars) and nucleus (open bars) after 3 and 12 h of incubation for the indicated siRNAs. Mean values ± SE.