Figure 8.

Nitrate Content in Shoot Is Increased in nrt1.9 Mutants.

(A) Nitrate content relative to fresh weight (FW) of shoots and roots in the wild type and nrt1.9 mutants.

(B) The shoot-to-root ratio of the nitrate content. Wild-type plants and nrt1.9 mutants were grown in liquid medium with 10 mM NH₄NO₃ for 10 d, and the nitrate contents in both shoot and root were measured by HPLC.

Values in (A) and (B) are mean ± se for 4 replicates, and each replicate contained 30 seedlings. An asterisk indicates a significant difference (P < 0.05) compared with the values of the wild type. Similar results were shown in three independent experiments.