Expression of Osmotic Stress–Responsive Genes in ced1 and Wild-Type Plants.
The qRT-PCR analysis was performed with total RNA from ced1 and wild-type (WT) plants under untreated control or osmotic stress treatment (40% PEG, 6 h) conditions. Real-time RT-PCR quantifications were normalized to the expression of TUB8. Error bars represent se from three independent experiments.
(A) qRT-PCR analysis of representative stress-responsive genes.
(B) Confirmation by qRT-PCR of the expression of genes showing significant changes between the wild type and the ced1 mutant in microarray analysis.