Experimental Setup.
(A) Schematic representation of leaf 3 development with proliferating (P; red), expanding (E; green), and mature (M; white) cells. At 9 DAS, plants were transferred to mannitol, and leaf 3 was dissected for growth, ploidy, and molecular analysis.
(B) Nine-day-old seedling.
(C) Electron micrograph of the 3rd and 4th leaves at 9 DAS.
Bars = 2 mm in (B) and 200 μm in (C).