Figure 4.

Effects of Varying Duration of Stress on Proliferating Leaves.

Plants were transferred to mannitol-containing plates at 9 DAS and then transferred back to control plates after 10 h (10M), 24 h (24M), or 48 h (48M) of mannitol treatment or kept on mannitol plates (M). Leaf 3 was dissected for further analysis.

(A) Reduction of leaf area at 10 DAS (24 h after first transfer).

(B) Reduction of leaf area and cell number at 14 DAS.

(C) Ploidy analysis. EI stands for endoreduplication index and represents the average number of endocycles undergone by a typical nucleus.

Data in (A) to (C) show means ± se from three independent experiments with multiple leaves measured in each experiment.