Figure 7.

Rapid Increase in ACC Levels after Stress Imposition.

(A) Heat map of selected ethylene signaling genes differentially regulated by osmotic stress in the 3rd fully proliferating leaf 1.5, 3, 12, and 24 h after stress imposition. Data are from Affymetrix ATH1 arrays and are expressed as the log₂ of fold change (mannitol, control). Red and green indicate upregulation and downregulation, respectively. A key to the gene names is provided in the Supplemental Table 3 online.

(B) ACC levels determined in shoots of 9-DAS seedlings 1 and 10 h after transfer to mannitol. Data show means ± se of ~100 to 150 plants from three independent experiments. Asterisk indicates significance (t test, P value < 0.05).