Figure 2.

In Planta Protein Interaction of FIT and EIN3/EIL1.

BiFC of YFP in transiently transformed tobacco leaf epidermal cells. Left column ([A], [D], [G], [J], and [M]), YFP fluorescent signal detection by confocal microscopy; middle column ([B], [E], [H], [K], and [N]), differential interference contrast (DIC) microscopy; right column ([C], [F], [I], [L], and [O]), merge of fluorescent signal and DIC. YC-FIT plus YN-EIN3 ([A] to [C]); YC-FIT plus YN-EIL1 ([D] to [F]); negative control YC plus YN-EIN3 ([G] to [I]); YC-FIT plus negative control YN ([J] to [L]); negative control YC plus negative control YN ([M] to [O]). For details on the number of experiments and fluorescence signals, refer to Methods.