Figure 5.

Transcriptome Changes between ein3 eil1 Mutants and the Wild Type at + and –Fe.

The Venn diagrams represent the number of genes that were found to be significantly differentially expressed between ein3 eil1 and wild-type (WT) seedlings grown in the 6-d growth assay, upon – and +Fe (total number of differentially expressed genes was 101 out of a total of 22,089 genes, compare with gene list in Supplemental Data Set 1 online). The upper Venn diagram represents the number of genes that were found to be downregulated, and the lower Venn diagram depicts the number of genes that were found to be upregulated in the comparison of ein3 eil1 versus the wild type. Genes in dark gray have assigned functions in the ethylene signaling pathway. Genes in medium gray are markers for a differential regulation between − and +Fe in the wild type. Genes in light gray have an assigned function in the chloroplast and light responses. Differential expression of EBF2 and FRO2 was verified in RT-PCR experiments using the same RNA samples used for microarray hybridization (see Supplemental Figure 3 online).