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. 2011 May 17;23(5):1815–1829. doi: 10.1105/tpc.111.084715

Figure 7.

Figure 7.

FIT Protein Regulation in ein3 eil1 and upon MG132 Treatment.

(A) FIT protein abundance in seedling roots of the wild type (WT) and ein3 eil1, exposed to + or –Fe as indicated, showing that FIT protein abundance is reduced in the ein3 eil1 mutant.

(B) The effect of MG132 on FIT abundance in AVG-treated roots, showing that MG132 treatment restored FIT abundance upon AVG treatment. Wild-type seedlings, exposed to + and −Fe, treated with 10 μM AVG or untreated (co, control) and treated for 4 h with or without 100 μM MG132 (+ or −MG132).

(C) The effect of MG132 on FIT abundance in ein3 eil1, showing that MG132 restored FIT protein levels in ein3 eil1 mutants. Seedlings exposed to + and −Fe, treated for 4 h with or without 100 μM MG132 (+ or –MG132); immunoblot analysis with anti-FIT-C antibody (top image; asterisk indicates 35-kD position of FIT protein) and Ponceau S-staining of proteins on the same membrane (bottom image).