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. 2011 May 20;23(5):1729–1740. doi: 10.1105/tpc.111.083915

Figure 3.

Figure 3.

Posttranscriptional Regulation and miR163 Biogenesis of At and Aa pri-miR163 Precursors in A. thaliana and Allotetraploids.

(A) Small RNA gel blot analysis of total RNA from leaves of 4-week-old transgenic A. thaliana that overexpressed At pri-miR163 and Aa pri-miR163. The two separate blots had the same exposure time. Numbers correspond to different independent transgenic lines.

(B) Small RNA gel blot analysis of total RNA from leaves of 4-week-old transgenic allotetraploids overexpressing 24-nucleotide (nt) At or 23-nucleotide Aa miR163. Numbers below the blots indicate relative densitometric intensity ratios of miR163 abundance in the transgenic lines compared with the endogenous AtmiR163 level in Allo3, which were normalized to U6 and Allo3. The numbers above the gel correspond to individual transgenic plants.