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Immunological Analysis of PSII Complexes Separated by BN-PAGE.
(A) A representative immunoblot with anti-D1 antiserum of a BN-PAGE gel. Thylakoid membrane proteins were solubilized with 2% dodecyl β-d-maltoside and separated by BN-PAGE. A sample with an equal amount of chlorophyll (1.5 μg) was loaded in each lane. GL, growth light; AHL, after 2 d of high light; WT, wild type.
(B) A representative immunoblot with anti-CP43 antiserum of a BN-PAGE gel as in (A).
