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Analysis of LQY1 Protein in Grana-, Grana Margin-, and Stroma Lamellae-Enriched Thylakoid Membranes.
Thylakoid membranes were solubilized by 0.4% digitonin and fractionated by ultracentrifugation. Fractionated thylakoid membrane proteins were separated by SDS-Urea-PAGE and analyzed by immunoblots. The lanes on each gel were loaded on an equal chlorophyll basis. GL, growth light; AHL, after 2-d high light; WT, wild type.
