Figure 1.

(a) Immunoblotting for the detection of megalin, in the pooled urine of DBA/2J mice without diabetes (CON) or with diabetes (STZ) for 10-, 15-, or 18-week duration (n = 6 mice/group, containing equal amounts of creatinine (Cr) for each sample (2.0 μg Cr/lane)). (b) Megalin gene expression as determined by qRT-PCR in renal tissue from control and diabetic DBA/2J mice at 10-, 15-, and 18-week duration. (c) Correlation of megalin and cubilin gene expression in kidneys from diabetic DBA/2J mice (10, 15, and 18 weeks). Immunoblotting and qRT-PCR were carried out as described in Section 2.