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. 2011 May 16;2011:269378. doi: 10.1155/2011/269378

Figure 2.

Figure 2

(a) Immunoblotting for the detection of VDBP in the pooled urine of DBA/2J mice without diabetes (CON) or with diabetes (STZ) for 10-, 15-, or 18-week duration (n = 6 mice/group, containing equal amounts of creatinine (Cr) for each sample (2.0 μg Cr/lane)). (b) VDBP in urine of control and diabetic mice was assessed by semiquantitative dot blotting, and urine 25-OHD was assayed by ELISA in urine of control and diabetic mice as described in Section 2. (c) Correlation of VDBP and 25-OHD assayed in urine from diabetic DBA/2J mice (10, 15, and 18 weeks—see legend).