Figure 1.

C. trachomatis-infected endocervical epithelial cell (A2En) culture. The immortalized endocervical epithelial cell line A2EN was exposed to C. trachomatis serovar D at multiplicity of infection of 2 in the absence of cyclohexamide (right two panels). This resulted in a mixed population of infected and uninfected cells. Infected cells are stained green with chlamydial LPS conjugated to FITC, and nucleic acid is stained with DAPI (blue). Uninfected controls are included in the left two panels for comparison. Data is representative of experiments performed greater than 10 times.