Figure 2. Identification of EYFP⁺ Neural Stem Cells.

Confocal micrographs depict EYFP⁺ cells with radial (A, E, I, M) morphology. These EYFP⁺ radial cells also express GFAP (B,F,J,N) in the radial process (D, H, L, P). Nestin⁺EYFP⁺ cells are almost all GFAP⁺ and exhibit radial morphology 6 days after TMX (A–D, S). EYFP⁺GFAP⁺ radial cells co-label with the stem cell marker BLBP (G, H), but not with the non-stem astrocyte marker S100β (K, L). Non-radial, stellate cells co-label with S100β and GFAP (L, white arrow). EYFP⁻GFAP⁺BLBP⁺ radial cells and EYFP⁻GFAP⁺S100β⁺ stellate cells are readily detected (H, L). However, nearly all EYFP⁺GFAP⁺ radial cells also express BLBP (Q), while almost none express S100β (R). Some EYFP⁺GFAP⁺ radial cells co-label with the cell division marker MCM2 (O, P)