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. Author manuscript; available in PMC: 2012 Jun 9.
Published in final edited form as: Neuron. 2011 Jun 9;70(5):908–923. doi: 10.1016/j.neuron.2011.05.022

Figure 3. Defining the cellular components of the EYFP+ neural stem cell lineage.

Figure 3

(A–E) Confocal micrographs reveal that EYFP+ cells (A) also express GFAP (B,C), DCX (D), and NeuN (E). EYFP+GFAP+ cells display morphology typical of either NSCs (B) or stellate astrocytes (C). DCX+ immature neurons (D) and NeuN+DCX mature neurons (D,E) are also present within the EYFP+ lineage. (F) Fate mapping analysis of EYFP+ cells following a brief TMX administration protocol reveals that GFAP+DCXNeuN NSCs are the predominant cell type present 5 days after TMX and progressively decrease as GFAPDCX+ cells appear over time. EYFP+GFAPDCXNeuN round cells are also present at all time points.