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. 2011 Jun;178(6):2888–2896. doi: 10.1016/j.ajpath.2011.02.013

Figure 2.

Figure 2

Effect of GTN on the secretion and expression of sFlt-1 and sEng by chorionic villi explants exposed to hypoxia. A and B: Relative sFlt-1 and sEng levels, respectively, in the medium of explant cultures (six explants per placenta, with a minimum of three placentas per treatment). Concentrations (in ng/mL) were divided by the total amount of protein (in μg/mL) in the media. Error bars represent SEM. The lines in A and B identify the comparison groups by the vertical lines pointing to the corresponding bars. C and D: Localization of Flt-1, Eng, cytokeratin (trophoblast marker), and nuclei (DAPI) by fluorescence microscopy. Far right: The merged images with all three markers combined, with yellow representing areas of colocalization of sFlt-1 or Eng with cytokeratin, are shown. Top: Explants exposed to hypoxia (0.5% O2). Bottom: Explants exposed to hypoxia in the presence of 1 μmol/L GTN. *P < 0.05, **P < 0.01, and ***P < 0.001. Transverse lines link two groups to indicate statistical significance. Scale bars = 10 μm. ELISA indicates enzyme-linked immunosorbent assay.