Figure 3.

Effect of GTN on the hypoxia-induced accumulation of HIF-1α in chorionic villi explants. A: Western blot analysis of HIF-1α protein in explants cultured in 20%, 8%, or 0.5% O₂. The administration of GTN (1 μmol/L or 10 nmol/L) inhibited the accumulation of HIF-1α in explants incubated in 0.5% O₂. Incubation of explants in 8% O₂ did not lead to accumulation of HIF-1α. B: Densitometric quantification of pooled Western blots from three independent experiments. The lines in A and B identify the comparison groups by the vertical lines pointing to the corresponding bars. *P < 0.05, **P < 0.01, and ***P < 0.001. (Minimum of three placentas per treatment).