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. 2011 Jun;178(6):2888–2896. doi: 10.1016/j.ajpath.2011.02.013

Figure 4.

Figure 4

Effect of HIF-1α knockdown on the hypoxia-induced increase in mRNA levels and secretion of sFlt-1 and sEng by third-trimester chorionic villi explants. (minimum of three placentas per treatment). A: Expression of HIF-1α transcript levels relative to β-actin transcript levels as determined by real-time quantitative PCR (qPCR). B and C: Relative levels of sFlt-1 and sEng protein in the tissue culture media. Values (in ng/mL) were divided by the total amount of protein (in μg/mL) in the media. The lines in A and B identify the comparison groups by the vertical lines pointing to the corresponding bars. *P < 0.05, **P < 0.01, and ***P < 0.001. Lines with asterisks denote the comparison groups. ELISA indicates enzyme-linked immunosorbent assay.