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. 2011 Jun;178(6):2641–2653. doi: 10.1016/j.ajpath.2011.02.044

Figure 3.

Figure 3

Proteolytic cleavage of caspase-3 and cytoskeletal substrates in fascia tissue. A: Representative immunoblot analyses from non-IH and IH fascia. Pan-actin was used as a loading control for normalization purposes. MW, molecular weight. B: Densitometric analysis of native and cleaved protein expression, in non-IH (n = 8) and IH (n = 8) samples (ns, nonsignificant; *P < 0.05, **P < 0.01, ***P < 0.001; Student's t-test). C: Representative immunoblots for the cytosol- (soluble) and cytoskeletal-enriched fractions from non-IH and IH fascia homogenates. D: Representative immunoblots of cytosol-enriched fractions, after immunoprecipitation with a specific polyclonal anti-phospho-Ser/Thr antibody (details in the Materials and Methods section).