Figure 6.
Analysis of primary fibroblast behavior. Proliferation assays: (A) [3H]-thymidine and (B) 5-bromo-2′-deoxy-uridine (BrdU) incorporation into DNA, in non-IHFs (n = 5) and IHFs (n = 5). Abs, absorption. Migration and adhesion assay: (C and E) representative images of the in vitro scratch assay captured at 0 hours to 24 hours postwounding, in non-IHFs and IHFs, and comparison of wound closure percentage between non-IHFs (n = 5) and IHFs (n = 5) (details in the Materials and Methods section). D: BrdU in situ immunostaining. Magnification: ×100, ×600. F: Cell adhesion of non-IHFs (n = 5) and IHFs (n = 5), quantified by inverted centrifugation. G: Migration of non-IHFs (n = 5) and IHFs (n = 5) across fibronectin- and type I collagen-coated inserts in transwell assays. *P < 0.05, **P < 0.01