Skip to main content
. 2011 Jun;178(6):2641–2653. doi: 10.1016/j.ajpath.2011.02.044

Figure 8.

Figure 8

LC3 immunoblots in fascia tissue. Representative immunoblots of LC3-I/LC3-II in whole lysates (RIPA lysis buffer), cytosolic- and membrane-enriched fractions (ProteoExtract Subcellular Proteome Extraction Kit) from non-IH and IH fascia tissues. SDS-PAGE was performed using 12.5% polyacrylamide gels and positive controls for anti-LC3 antibody (MBL). The cytosolic and membrane protein fractions were further concentrated to about sevenfold on Amicon Ultra 0.5-mL centrifugal filters (Millipore) and resolved in 11% and 8% SDS polyacrylamide gels, respectively. β-Tubulin detection in cytosolic protein extracts was used as a loading control for normalization purposes. Flotillin-2 was used as a membrane marker.