Fig. 7.

Loss of Acvr1 leads to proliferation in posterior subcapsular plaques, forming ‘lens tumors’. P22 lenses were stained with the nucleic acid stain TOTO-1 and their posterior poles were examined using a confocal microscope. The inset in A shows the plane of section and region of the lens that was viewed. (A) No nuclei were seen in the posterior poles of Acvr1^WT lenses. Staining of RNA in the fiber cell cytoplasm shows the posterior lens sutures. (B) A few scattered nuclei were evident near the posterior surface of Acvr1^CKO lenses. (C) A small accumulation of nuclei was present at the posterior pole of Trp53^CKO lenses. (D) A large mass of cells was present at the posterior poles of Acvr1;Trp53^DCKO lenses. (E) Antibody to Ki67 showed that cells in the posterior subcapsular plaques of Trp53^CKO lenses were not in the cell cycle. (F) Antibody to Ki67 showed that cells in the posterior subcapsular plaques of Acvr1;Trp53^DCKO lenses were still in the cell cycle.