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. 2011 Jun;178(6):2823–2831. doi: 10.1016/j.ajpath.2011.02.046

Figure 4.

Figure 4

Axonal degeneration and demyelination of optic nerves during EAE. A–D: Representative images of optic nerve cross-sections stained using Bielschowsky's silver impregnation to label axons at EAE day 21 in WT-Prnp (A), Prnp−/− (B), WT-Tg35 (C), and Tg35 (D) mice. B: Red arrow indicates an axon, and white arrow, a macrophage. E: Quantification of the number of surviving axons at various stages of EAE. Significantly decreased numbers of axons were present in Prnp−/− mice at EAE days 1 and 8, and in Tg35 mice at EAE days 1, 8, and 21, as compared with their respective WT counterparts. F–I: Representative images of optic nerve cross-sections at EAE day 21 stained using Luxol fast blue to label myelin in WT-Prnp (F), Prnp−/− (G), WT-Tg35 (H), and Tg35 (I) mice. J: Quantification of the percentage of demyelination of optic nerves showed significantly higher demyelination in Prnp−/− mice on EAE day 8 and in Tg35 mice at EAE days 1, 8, and 21. Scale bars: 20 μm (A–D); 100 μm (F–I). *P < 0.05. **P < 0.01.